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https://doi.org/10.53941/rmd.2025.100005
Liu, B., Mu, Y., & Wang, D.-A. hiPSC-Driven Organoid Construction and Application Prospects. Regenerative Medicine and Dentistry. 2025, 2(1), 5. doi: https://doi.org/10.53941/rmd.2025.100005
Volume 2, Issue 1, 2025
Copyright (c) 2025 by the authors.
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This work is licensed under a Creative Commons Attribution 4.0 International License.

Perspective

hiPSC-Driven Organoid Construction and Application Prospects

Bangheng Liu 1,2, Yulei Mu 2,3 and Dong-An Wang 1,2,*

1 Department of Biomedical Engineering, Chinese University of Hong Kong, Sha Tin, New Territories, Hong Kong SAR 999077, China

2 Center for Neuromusculoskeletal Restorative Medicine, InnoHK, HKSTP, Sha Tin, New Territories, Hong Kong SAR 999077, China

3 Department of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR 999077, China

* Correspondence: donganwang@cuhk.edu.hk

Received: 5 March 2025; Revised: 19 March 2025; Accepted: 20 March 2025; Published: 21 March 2025

Abstract: Induced pluripotent stem cell (iPSC)-derived organoid platforms can simulate various target tissues and hold broad application prospects in personalized medicine, disease modeling, drug screening, organ transplantation, and understanding organ development mechanisms. Currently, the development of human iPSC (hiPSC) organoids is gradually shifting towards Matrigel-free and scaffold-free systems, promoting precise control over the composition and structure of these systems and establishing induction protocols for specialized organoids. Researchers are also exploring the construction of multifunctional systems with complex structures and material exchange channels through vascularization, segmented induction, and assembly technologies, though further breakthroughs are needed. In the future, hiPSC organoids are expected to advance towards personalized precision treatment, high-throughput module detection systems, multi-organ integration, and automation. Additionally, when combined with large artificial intelligence models, there is potential to establish hiPSC data and medical platforms, providing support for drug development and clinical decision-making. Moreover, the development of medical AI is anticipated to foster collaboration rather than competition, promoting coordinated growth in the field. For hiPSC-derived platforms, it is crucial to further enhance the ethical review framework to balance radical scientific exploration with conservative public attitudes. Researchers must also optimize or develop new induction protocols to reduce genomic instability and tumorigenic risks, while avoiding the emergence of non-target cells and insufficient functional maturity.

Keywords:

hiPSC organoid multi-organ integration vascularization technology AI personalized medicine

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